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biotinylated goat anti guinea pig secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat anti guinea pig secondary antibody
    Biotinylated Goat Anti Guinea Pig Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+guinea+pig+secondary+antibody/Biotinylated+Goat+Anti-Guinea+Pig+IgG+Antibody/pmc13043728-280-6-20
    Average 95 stars, based on 973 article reviews
    biotinylated goat anti guinea pig secondary antibody - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Labeling:

    Article Title: Activity-dependent synaptic GRIP1 accumulation drives synaptic scaling up in response to action potential blockade
    Article Snippet: Neurons were plated on gridded coverslips under normal culture conditions (catalog no. P35G-2-14-GCRD; Matek); 7- to 9-DIV pyramidal neurons were identified based on morphology and their location on the dish was noted. .. Cells were fixed in 4% paraformaldehyde, labeled with guinea pig anti-VGluT1 antibody (1:600, catalog no. 135 304; Synaptic Systems) followed by biotinylated anti-guinea pig secondary antibody (1:400, catalog no. BA-7000; Vector Labs) and then immersed in a 1% DAB solution in the presence of H 2 O 2 solution for 1–2 min. Next neurons were dehydrated through a series of ethanol and acetone mixtures. ..

    Amplification:

    Article Title: Muscle Cell Fate Choice Requires the T-Box Transcription Factor Midline in Drosophila
    Article Snippet: Colorimetric detection used biotinylated antimouse secondary antibodies (1:200, Vector Laboratories) and the Vectastain ABC Elite kit (Vector Laboratories). .. Fluorescent signal amplification used biotinylated anti-guinea pig secondary antibody and biotinylated antichicken secondary antibody (1:500, Vector Laboratories) and followed the protocol as described in the TSA kit (Tyramide Signal Amplification kit from Life Technologies). .. Confocal microscopy and image processing For , , , , , , and Supporting Information , Figure S2 , Figure S5 , and Figure S6 , confocal microscopy was performed on fixed embryos using Zeiss LSM 510 VIS and Zeiss LSM 700 Falcon microscopes with a Plan-Apochromat ×40/1.3 oil DIC objective.

    Incubation:

    Article Title: TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice
    Article Snippet: Sections were then incubated with a blocking solution containing 2.5% normal horse serum (PK-7200, Vectastain Elite ABC-HRP Kit, Vector Laboratories, Burlingame, USA), and incubated with the following primary antibodies: RNA-binding protein with multiple splicing (RBPMS, #1832, guinea pig polyclonal, 1:500, PhosphoSolutions, Colorado, USA); glutamic acid decarboxylase (GAD67, #ab26116, mouse monoclonal [K-87], 1:100, Abcam, Cambridge, UK); neuronal nuclei (NeuN, #MAB377, mouse monoclonal [A60], 1:250, Merck, Milan, Italy); F4/80 (#MA516624, rat monoclonal [Cl:A3-1], 1:50, Thermo Fisher Scientific, Rockford, USA) diluted in antibody diluent (Roche Diagnostics, Mannheim, Germany) for 1 h at room temperature. .. Sections were then incubated with prediluted biotinylated anti-mouse/rabbit IgG secondary antibody or with biotinylated anti-guinea pig secondary antibody (1:200, #BA-7000, Vector Laboratories, Burlingame, USA), 30 min at room temperature, and in avidin-biotin complex solution (#PK-7200, Vectastain Elite ABC-HRP Kit, Vector Laboratories, Burlingame, USA) for 30 min at room temperature. .. The sections were then transferred to peroxidase substrate (#K3468, ImmPACT DAB, Vector Laboratories, Burlingame, USA) for 4–6 min for the chromogen development reaction and rinsed in distilled water before mounting.

    Article Title: Long-lasting effects of early-life intervention in mice on adulthood behaviour, GABA A receptor subunit expression and synaptic clustering.
    Article Snippet: Variations in the early postnatal environment of rodents produce long-term changes in responses to stress that may underlie neuropsychiatric diseases such as anxiety, depression and schizophrenia.. GABAA receptors undergo marked changes in their subunit composition during this period, involving a regionally-dependent replacement of 2 with 1 subunits, the so-called -subunit switch.. In this study we examined the effects of early-life environment on adulthood GABAA receptor 1 and 2 subunit expression and the synaptic clustering of GABAA receptors.

    Article Title: Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25
    Article Snippet: Sections were incubated in PBS containing 3% BSA (Sigma-Aldrich, A9647) (blocking solution) for 2 h, followed by incubation with guinea pig anti-Vesicular Glutamate Transporter 1 (VGluT1) antibody (1:2000, Millipore, AB5905) in the blocking solution for overnight at 4 °C. .. Staining was visualized by incubation with biotinylated anti-guinea pig secondary antibody (1:500, Vector Laboratories, BA7000) for 2 h and then with Alexa 488 (1:500, Invitrogen, S32354) or Cy5 (1:500, Invitrogen, SA1011) streptavidin-conjugated antibody for 2 h at room temperature (RT). ..

    Avidin-Biotin Assay:

    Article Title: TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice
    Article Snippet: Sections were then incubated with a blocking solution containing 2.5% normal horse serum (PK-7200, Vectastain Elite ABC-HRP Kit, Vector Laboratories, Burlingame, USA), and incubated with the following primary antibodies: RNA-binding protein with multiple splicing (RBPMS, #1832, guinea pig polyclonal, 1:500, PhosphoSolutions, Colorado, USA); glutamic acid decarboxylase (GAD67, #ab26116, mouse monoclonal [K-87], 1:100, Abcam, Cambridge, UK); neuronal nuclei (NeuN, #MAB377, mouse monoclonal [A60], 1:250, Merck, Milan, Italy); F4/80 (#MA516624, rat monoclonal [Cl:A3-1], 1:50, Thermo Fisher Scientific, Rockford, USA) diluted in antibody diluent (Roche Diagnostics, Mannheim, Germany) for 1 h at room temperature. .. Sections were then incubated with prediluted biotinylated anti-mouse/rabbit IgG secondary antibody or with biotinylated anti-guinea pig secondary antibody (1:200, #BA-7000, Vector Laboratories, Burlingame, USA), 30 min at room temperature, and in avidin-biotin complex solution (#PK-7200, Vectastain Elite ABC-HRP Kit, Vector Laboratories, Burlingame, USA) for 30 min at room temperature. .. The sections were then transferred to peroxidase substrate (#K3468, ImmPACT DAB, Vector Laboratories, Burlingame, USA) for 4–6 min for the chromogen development reaction and rinsed in distilled water before mounting.

    Next-Generation Sequencing:

    Article Title: Long-lasting effects of early-life intervention in mice on adulthood behaviour, GABA A receptor subunit expression and synaptic clustering.
    Article Snippet: Variations in the early postnatal environment of rodents produce long-term changes in responses to stress that may underlie neuropsychiatric diseases such as anxiety, depression and schizophrenia.. GABAA receptors undergo marked changes in their subunit composition during this period, involving a regionally-dependent replacement of 2 with 1 subunits, the so-called -subunit switch.. In this study we examined the effects of early-life environment on adulthood GABAA receptor 1 and 2 subunit expression and the synaptic clustering of GABAA receptors.

    Staining:

    Article Title: Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25
    Article Snippet: Sections were incubated in PBS containing 3% BSA (Sigma-Aldrich, A9647) (blocking solution) for 2 h, followed by incubation with guinea pig anti-Vesicular Glutamate Transporter 1 (VGluT1) antibody (1:2000, Millipore, AB5905) in the blocking solution for overnight at 4 °C. .. Staining was visualized by incubation with biotinylated anti-guinea pig secondary antibody (1:500, Vector Laboratories, BA7000) for 2 h and then with Alexa 488 (1:500, Invitrogen, S32354) or Cy5 (1:500, Invitrogen, SA1011) streptavidin-conjugated antibody for 2 h at room temperature (RT). ..



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